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Extraction of Bioactive Compounds from C. vulgaris Biomass Using Deep Eutectic Solvents
Maria Myrto Dardavila1, Sofia Pappou1,2, Maria G Savvidou1
1Laboratory of Thermodynamics and Transport Phenomena, Zografou Campus, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., 15780 Athens, Greece.
Molecules (Basel, Switzerland)
|January 8, 2023
Summary
Choline chloride-based deep eutectic solvents efficiently extracted bioactive compounds from Chlorella vulgaris microalgae. The optimal solvent, ChCl:1,2 butanediol/water, was identified for maximizing carotenoid and phenolic content.
Area of Science:
- Biotechnology
- Green Chemistry
- Phytochemistry
Background:
- Microalgae like *C. vulgaris* are rich sources of valuable bioactive compounds.
- Deep Eutectic Solvents (DESs) offer a sustainable alternative for extracting these compounds.
- Choline Chloride (ChCl) based DESs have shown promise in biomass processing.
Purpose of the Study:
- To screen and identify the most effective Choline Chloride (ChCl) based Deep Eutectic Solvents (DESs) for extracting bioactive compounds from *C. vulgaris*.
- To optimize the extraction process using the most efficient solvent and Response Surface Methodology (RSM).
- To determine the impact of solvent-to-biomass ratio, temperature, and time on total carotenoid content (TCC), total phenolic content (TPC), and antioxidant activity (IC50).
Main Methods:
- Extraction of bioactive compounds from *C. vulgaris* using various ChCl-based DESs mixed with water.
- Quantification of total carotenoid content (TCC) and total phenolic content (TPC).
- Assay of antioxidant activity (IC50).
- Optimization of extraction parameters (solvent-to-biomass ratio, temperature, time) using Response Surface Methodology (RSM).
Main Results:
- ChCl:1,2 butanediol (1:4)/H2O (70/30 w/w) was identified as the most effective solvent for extraction.
- RSM models were developed to predict TCC, TPC, and IC50 based on extraction parameters.
- Experimental validation confirmed the predictive capacity of the developed models.
Conclusions:
- ChCl:1,2 butanediol/water is a highly efficient DES for extracting bioactive compounds from *C. vulgaris*.
- The optimization study provides a robust method for maximizing the yield of valuable compounds.
- This research contributes to sustainable microalgae biomass valorization through green extraction techniques.
