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DNA-Directed Protein Anchoring on Oligo/Alkanethiol-Coated Gold Nanoparticles: A Versatile Platform for Biosensing
Ahmed Alsadig1,2, Behnaz Abbasgholi-Na2,3, Hendrik Vondracek2
1Department of Physics, University of Trieste, 34127 Trieste, Italy.
Nanomaterials (Basel, Switzerland)
|January 8, 2023
Summary
A novel biosensing platform uses gold nanoparticles and DNA-protein conjugates for sensitive antigen detection. This smart platform, employing miniaturized gel electrophoresis with thermal lens spectrometry, can detect cancer-associated antigens like HER2-ECD.
Area of Science:
- Nanotechnology
- Biotechnology
- Analytical Chemistry
Background:
- Biosensors are crucial for disease detection.
- Gold nanoparticles (AuNPs) offer unique optical properties for biosensing.
- Sensitive detection of cancer biomarkers is a significant challenge.
Purpose of the Study:
- To develop a smart biosensing platform for high-sensitivity antigen detection.
- To functionalize AuNPs with ssDNA and DNA-protein conjugates.
- To utilize a novel MGEC-TLS technique for antigen quantification.
Main Methods:
- Functionalization of AuNPs with ssDNA and bio-repellent molecules (TOEG6).
- DNA-directed immobilization (DDI) of DNA-protein conjugates (C8 nanobody).
- Detection of HER2-ECD antigen using miniaturized gel electrophoresis integrated with online thermal lens spectrometry (MGEC-TLS).
Main Results:
- Characterization of AuNPs confirmed colloidal stability and optimal surface properties.
- The biosensing platform demonstrated high sensitivity for HER2-ECD detection.
- MGEC-TLS detected HER2-ECD down to 440 ng/mL, outperforming conventional methods.
Conclusions:
- The developed biosensing platform is versatile and sensitive.
- DNA hybridization on plasmonic scaffolds enables efficient immobilization.
- The MGEC-TLS technique offers a sensitive, label-free approach for disease-relevant antigen detection.
