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Double-barrel pipette system for microinjection.

M J Davis¹, R W Gore

¹Department of Medical Physiology, Texas A&M University, College Station 77843.

The American Journal of Physiology

|October 1, 1987

Summary

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This study details a novel method for creating double-barrel micropipettes using theta-tubing. These specialized pipettes allow for independent dual-channel operation, ideal for precise biological experiments.

Area of Science:

Biomedical Engineering
Neuroscience
Physiology

Background:

Micropipettes are essential tools in biological research for precise fluid handling.
Existing micropipette designs can present challenges in fabrication and simultaneous dual-channel functionality.

Purpose of the Study:

To describe a new method for constructing double-barrel micropipettes.
To highlight the advantages of using theta-tubing for pipette fabrication.
To demonstrate the utility of these micropipettes in various physiological measurements.

Main Methods:

Construction of double-barrel micropipettes utilizing theta-tubing.
Easy beveling of pipette tips facilitated by the theta-tubing material.
Independent operation of the two pipette barrels.

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Main Results:

Successfully fabricated double-barrel micropipettes with easily beveled tips.
Demonstrated independent functionality of the two pipette channels.
Confirmed suitability for simultaneous microinjection, microperfusion, and servo-null micropressure measurements.

Conclusions:

The described method provides an efficient way to construct versatile double-barrel micropipettes.
Theta-tubing simplifies pipette fabrication and enhances tip manipulation.
These micropipettes offer a robust solution for advanced biological and physiological research applications.