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Interaction study of aflatoxin M1 with milk proteins using ATR-FTIR
C G Harshitha1, Neelima Sharma1, Richa Singh1
1Dairy Chemistry Division, NDRI, Karnal, India.
Abstract:
Aflatoxin M1 (AFM1) is a metabolite of carcinogenic aflatoxin B1 (AFB1) and appears in milk of dairy animals on ingestion of feed contaminated with AFB1. It has been reported to have affinity towards milk proteins, the exact mechanism of which is still unknown. In the present study, ATR-FTIR coupled with chemometrics is utilized to understand AFM1 interaction with milk proteins. The second order derivative spectra of the spectral window 1700-1600 cm-1 confirms the affinity of AFM1 towards milk proteins. The results of principal component analysis suggested that spectral window of 1700-1600 cm-1 is informative and provides an indication of the conformational changes brought by AFM1 in the secondary structure of milk proteins.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13197-022-05587-x.
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