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Cross-contamination risk and decontamination during changeover after cell-product processing.

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Minimizing cross-contamination in cell processing is crucial. UV irradiation effectively eliminates contaminants in biosafety cabinets, offering a promising method for safe cell changeover procedures.

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Area of Science:

  • Biotechnology
  • Microbiology
  • Cellular Biology

Background:

  • Minimizing cross-contamination during cell-product processing is essential.
  • Human error in manual processing increases contamination risks within biosafety cabinets.
  • Patient-specific cell origins influence cross-contamination risks.

Purpose of the Study:

  • To evaluate cross-contamination risks during manual cell processing.
  • To develop an evidence-based changeover method for biosafety cabinets.
  • To assess the efficacy of UV irradiation in mitigating contamination.

Main Methods:

  • Simulated medium preparation, cell seeding, and waste decanting were performed by operators of varying skill levels.
  • Environmental bacteria were surveyed across four facilities.
  • The impact of UV irradiation on bacteria and fungi was assessed.

Main Results:

  • Contamination spread via droplets and bubble ruptures up to 50 cm.
  • Bacillus subtilis, with resistant endospores, was detected in facilities.
  • UV irradiation at 50 mJ/cm² eliminated vegetative cells and fungi; 100 mJ/cm² eliminated endospores.

Conclusions:

  • UV irradiation effectively prevents cross-contamination under simulated optimal conditions.
  • Monitoring UV dose during biosafety cabinet changeover is a promising strategy.
  • This approach can significantly reduce cross-contamination risks in cell processing.