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Updated: Aug 14, 2025

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Published on: July 7, 2023
Cross-contamination risk and decontamination during changeover after cell-product processing
Mitsuru Mizuno1, Kouichirou Yori2, Toshikazu Takeuchi2
1Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University (TMDU), 1-5-45, Bunkyo-ku, Yushima, Tokyo 113-8519, Japan.
Minimizing cross-contamination in cell processing is crucial. UV irradiation effectively eliminates contaminants in biosafety cabinets, offering a promising method for safe cell changeover procedures.
Area of Science:
- Biotechnology
- Microbiology
- Cellular Biology
Background:
- Minimizing cross-contamination during cell-product processing is essential.
- Human error in manual processing increases contamination risks within biosafety cabinets.
- Patient-specific cell origins influence cross-contamination risks.
Purpose of the Study:
- To evaluate cross-contamination risks during manual cell processing.
- To develop an evidence-based changeover method for biosafety cabinets.
- To assess the efficacy of UV irradiation in mitigating contamination.
Main Methods:
- Simulated medium preparation, cell seeding, and waste decanting were performed by operators of varying skill levels.
- Environmental bacteria were surveyed across four facilities.
- The impact of UV irradiation on bacteria and fungi was assessed.
Main Results:
- Contamination spread via droplets and bubble ruptures up to 50 cm.
- Bacillus subtilis, with resistant endospores, was detected in facilities.
- UV irradiation at 50 mJ/cm² eliminated vegetative cells and fungi; 100 mJ/cm² eliminated endospores.
Conclusions:
- UV irradiation effectively prevents cross-contamination under simulated optimal conditions.
- Monitoring UV dose during biosafety cabinet changeover is a promising strategy.
- This approach can significantly reduce cross-contamination risks in cell processing.
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