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Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
Ensieh Khalkhal1, Mostafa Rezaei-Tavirani1, Nastaran Asri2
1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Middle East Journal of Digestive Diseases
|January 9, 2023
Summary
New diagnostic markers for celiac disease were identified. Tumor necrosis factor alpha-induced protein 3 (TNFAIP3), interleukin-23A (IL-23A), and granzyme B (GzmB) show promise for differentiating patients from healthy individuals.
Area of Science:
- Immunology
- Gastroenterology
- Biomarker Discovery
Background:
- Celiac disease pathogenesis involves inflammatory cytokines.
- Current diagnostic methods for celiac disease can be invasive.
- Need for accessible, rapid, and cost-effective diagnostic markers.
Purpose of the Study:
- To evaluate peripheral blood gene expression levels of specific inflammatory markers in celiac disease patients.
- To identify potential non-invasive biomarkers for celiac disease diagnosis.
- To assess the diagnostic value of selected genes in differentiating celiac disease patients from healthy controls.
Main Methods:
- Quantitative real-time PCR was used to measure mRNA expression levels.
- Peripheral blood mononuclear cells were collected from 30 celiac disease patients and 30 healthy subjects.
- Receiver operating characteristic (ROC) curve analysis was performed to evaluate diagnostic ability.
Main Results:
- Significant upregulation of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 was observed in celiac disease patients.
- TNFAIP3, IL23A, and GzmB demonstrated superior diagnostic value in distinguishing celiac disease patients.
- Elevated expression of these genes suggests their involvement in celiac disease.
Conclusions:
- TNFAIP3, IL23A, and GzmB are potential sensitive and useful biomarkers for celiac disease diagnosis.
- Further investigation is needed to confirm the diagnostic relevance of other identified genes.
- These findings may lead to improved non-invasive diagnostic strategies for celiac disease.
