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The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
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Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution
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Deciphering postnatal limb development at single-cell resolution.

Manman Gao1,2,3,4, Xizhe Liu3, Peng Guo1,3

  • 1Department of Orthopaedic Surgery, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China.

Iscience
|January 9, 2023
PubMed
Summary
This summary is machine-generated.

This study reveals novel progenitor cells and developmental pathways in early postnatal limb development using single-cell RNA sequencing. Findings offer new insights into limb development, regeneration, and osteoarthritis.

Keywords:
Biological sciencesOmicsTranscriptomics

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Area of Science:

  • Developmental Biology
  • Genomics
  • Orthopedics

Background:

  • Early postnatal limb development is crucial but understudied compared to embryonic stages.
  • This developmental period mirrors pathological changes in articular cartilage.

Purpose of the Study:

  • To systematically characterize the cellular landscape of early postnatal limb development.
  • To identify novel progenitor cells and developmental trajectories in the murine hindlimb.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) was employed.
  • 19,952 single cells from murine hindlimbs across 4 postnatal stages were analyzed.
  • Cellular heterogeneity was delineated to identify distinct cell populations and developmental branches.

Main Results:

  • Identified novel progenitor sub-clusters in articular cartilage and enthesis, marked by Cd34 and Ly6e.
  • Discovered three distinct cellular developmental branches in the growth plate, marked by Col10a1, Spp1, and Tnni2.
  • Explored representative transcriptomes, developmental patterns, and key regulatory mechanisms.

Conclusions:

  • This research expands the understanding of postnatal limb developmental biology.
  • The findings highlight interconnections between limb development, cartilage remodeling, and regeneration.
  • Provides a foundation for studying osteoarthritis pathogenesis and potential therapeutic targets.