Biochar amendments and climate warming affected nitrification associated N₂O and NO production in a vegetable field

Soil nitrification driven by ammonia-oxidizing microorganisms is the most important source of nitrous oxide (N₂O) and nitric oxide (NO). Biochar amendment has been proposed as the most promising measure for combating climate warming; both have the potential to regulate the soil nitrification process. However, the comprehensive impacts of different aged biochars and warming combinations on soil nitrification-related N₂O and NO production are not well understood. Here, 1-octyne and acetylene were used to investigate the relative contributions of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to potential nitrification-mediated N₂O and NO production from the fertilized vegetable soil with different aged biochar amendments and soil temperatures in microcosm incubations. Results demonstrated that AOB dominated nitrification-related N₂O and NO production across biochar additions and climate warming. Biochar amendment did not significantly influence the relative contribution of AOB and AOA to N₂O and NO production. Field-aged biochar markedly reduced N₂O and NO production via inhibiting AOB-amoA gene abundance and AOB-dependent N₂O yield while fresh- and lab-aged biochar produced negligible effects on AOB-dependent N₂O yield. Climate warming significantly increased N₂O production and AOB-dependent N₂O yield but less so on NO production. Notably, the relative contribution of AOB to N₂O production was enhanced by climate warming, whereas AOB-derived NO showed the opposite tendency. Overall, the results revealed that field-aged biochar contributed to mitigating warming-induced increases in N₂O and NO production via inhibiting AOB-amoA gene abundance and AOB-dependent N₂O yield. Our findings provided guidance for mitigating nitrogen oxide emissions in intensively managed vegetable production under the context of biochar amendments and climate warming.