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Updated: Aug 14, 2025

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
Long-read genome sequence data of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae 119
Dineshkumar K1, Tony Grace2, Ginny Antony1
1Department of Plant Science, School of Biological Sciences, Central University of Kerala, Tejaswini Hills, Periye, Kasaragod 671320 Kerala, India.
Abstract:
Bacterial blight of pomegranate caused by Xanthomonas citri pv. punicae (Xcp) is a major constraint on pomegranate production in India. Multiple incidences of 60-80% yield losses have been reported from major growing areas. The molecular mechanism of pomegranate susceptibility to this disease is yet to be unraveled. A complete genome sequence is required to identify the pathogen's virulence factors. The available genomes of the pathogen are not complete. We have used the PacBio platform to obtain long reads and assembled the Xcp-119 genome and it consists of 15 contigs with an N50 value of 1.65 Mb. The assembled genome is 5.4 Mb in size; further, our annotation analysis yielded 5088 genes, 58 RNAs, and 7 Transcription activator-like effectors (TALEs). The assembled genome is suitable for identifying the virulence factors of this pathogen. The data presented here will be helpful to the scientific community for various comparative genome analyses and to decipher the mode and factors of pathogenesis.
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