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A Quantitative Glycomics and Proteomics Combined Purification Strategy
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Comparison of two methods for ginsenosides quantitation.

Serban C Moldoveanu1, Huamin Gan1

  • 1R.J. Reynolds Tobacco Co, Winston-Salem, North Carolina, USA.

Journal of Separation Science
|January 10, 2023
PubMed
Summary
This summary is machine-generated.

A novel liquid chromatography-tandem mass spectrometry method using cesium adducts ([M + Cs]+) offers superior sensitivity for ginsenoside analysis compared to sodium adducts. This advancement enables precise quantification of ginsenosides in various ginseng products and supplements.

Keywords:
dietary supplementsenergy drinksginseng rootginsenosides

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Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Mass Spectrometry

Background:

  • Ginsenosides are key bioactive compounds in ginseng with diverse pharmacological effects.
  • Accurate quantification of ginsenosides is crucial for quality control of ginseng products and research.
  • Existing liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods face sensitivity limitations.

Purpose of the Study:

  • To compare two LC-MS/MS methods for ginsenoside analysis.
  • To evaluate a novel method utilizing cesium adducts ([M + Cs]+) against a conventional sodium adduct ([M + Na]+) method.
  • To assess the sensitivity and applicability of the cesium adduct method for ginsenoside quantification.

Main Methods:

  • Two LC-MS/MS methods were developed and compared, sharing the same chromatographic separation.
  • Method 1 used conventional multiple reaction monitoring (MRM) with [M + Na]+ molecular ions.
  • Method 2 employed an original approach using [M + Cs]+ molecular ions and Cs+ as fragment ions.

Main Results:

  • The [M + Cs]+ method demonstrated significantly higher sensitivity, detecting concentrations as low as 4 ng/ml with a signal-to-noise ratio ≥ 20.
  • Both methods successfully quantified eight specific ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf (S), Rg1, Rg2).
  • The [M + Cs]+ method showed potential for analyzing other ginsenosides and was applied to ginseng root, supplements, energy drinks, and ashwagandha.

Conclusions:

  • The novel LC-MS/MS method using [M + Cs]+ adducts provides enhanced sensitivity for ginsenoside analysis.
  • This method is suitable for quantifying ginsenosides in complex matrices like dietary supplements and beverages.
  • The cesium adduct approach offers a valuable tool for sensitive and accurate ginsenoside determination in various applications.