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Updated: Aug 14, 2025
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
Published on: March 17, 2016
Protocol for microbiota analysis of a murine stroke model
Adam Sorbie1, Rosa Delgado Jiménez1, Monica Weiler1
1Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, Munich, Germany.
Abstract:
Investigations on the microbiota in neurological diseases such as stroke are increasingly common; however, stroke researchers may have limited experience with designing such studies. Here, we describe a protocol to conduct a stroke microbiota study in mice, from experimental stroke surgery and sample collection to data analysis. We provide details on sample processing and sequencing and provide a reproducible data analysis pipeline. In doing so, we hope to enable researchers to conduct robust studies and facilitate identification of stroke-associated microbial signatures. For complete details on the use and execution of this protocol, please refer to Sorbie et al. (2022).1.
Insights
This study details a protocol for investigating the gut microbiome in mouse models of stroke. It covers everything from surgery and sample collection to data analysis, aiming to help researchers identify stroke-associated microbial signatures.
Area of Science:
- Neuroscience
- Microbiology
- Immunology
Background:
- Research into the role of the microbiota in neurological diseases, including stroke, is growing.
- Stroke researchers may lack specialized experience in designing and executing microbiota studies.
- Standardized protocols are needed to ensure robust and reproducible findings in this field.
Purpose of the Study:
- To provide a detailed protocol for conducting microbiota studies in experimental stroke models in mice.
- To guide researchers through the entire process, from stroke induction to data analysis.
- To facilitate the identification of microbial signatures associated with stroke.
Main Methods:
- Experimental stroke surgery in mice.
- Collection and processing of biological samples (e.g., feces, blood, tissue).
- Microbiota sequencing (e.g., 16S rRNA gene sequencing).
- Development of a reproducible bioinformatic pipeline for data analysis.
Main Results:
- A comprehensive protocol for stroke-associated microbiota research in mice is described.
- Detailed methods for sample handling, sequencing, and data analysis are provided.
- The protocol is designed to be reproducible and enable robust study outcomes.
Conclusions:
- This protocol empowers researchers to conduct rigorous investigations into the stroke microbiota.
- It aims to standardize methodologies, facilitating the discovery of stroke-associated microbial patterns.
- Adoption of this protocol can advance our understanding of the gut-brain axis in stroke.
