Extenders and cryosolutions for Grumata (Prochilodus vimboides) sperm preservation

Area of Science:

• Aquatic biology
• Fish reproduction
• Cryopreservation

Background:

• Prochilodus vimboides populations face decline due to habitat alteration, overfishing, urbanization, and pollution.
• Effective sperm preservation techniques are crucial for the conservation of this species.

Purpose of the Study:

• To assess the efficacy of various extender solutions for short-term Prochilodus vimboides sperm storage.
• To determine optimal cryopreservation protocols for Prochilodus vimboides sperm using different cryoprotectants.

Main Methods:

• Sperm motility was evaluated after short-term storage (0-72 hours at 4-6°C) in NaCl, glucose, BTS, and MIII solutions.
• Cryopreservation involved diluting sperm in extenders and combining with dimethylsulfoxide, methyl glycol, or ethylene glycol.
• Post-thaw analysis included sperm motility and oxidative stress markers (catalase, lipid peroxidation).

Main Results:

• The 5% BTS extender maintained sperm motility above 40% for up to 48 hours.
• Cryopreservation with 5% glucose and methyl glycol yielded higher sperm motility.
• These cryopreserved samples exhibited reduced catalase activity and lipid peroxidation.

Conclusions:

• Short-term storage of Prochilodus vimboides sperm is feasible for up to 48 hours using a 5% BTS extender solution.
• Optimal cryopreservation of Prochilodus vimboides sperm can be achieved with 5% glucose and methyl glycol.
• These findings support the development of assisted reproduction strategies for Prochilodus vimboides conservation.