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Updated: Aug 14, 2025
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Trehalose in extenders for cryopreservation of Tambaqui (Colossoma macropomum) sperm
A S Varela Junior1, R D Jardim1, D P Streit2
1RAC- Reproducao Animal Comparada, Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Background:
Sugars may act as either energy substrates or non-penetrating cryoprotectants.
Objective:
Inclusion of non-penetrating trehalose was tested in extenders for the cryopreservation of Tambaqui (Colossoma macropomum) sperm.
Materials And Methods:
Sperm was extended 1/9 (v/v) in Beltsville Thawing Solution (BTS) with 10% DMSO (control) or 50, 100, 150 and 200 mM trehalose without 10% DMSO. Post-thawed sperm quality was evaluated, including fertilization and hatching rates, sperm motility, motility period and viability, integrity of sperm membrane and DNA, and mitochondrial functionality.
Results:
Extenders with 100 - 150 mM trehalose achieved fertilization and hatching rates similar to those of the 10% DMSO-treated sperm samples. Trehalose at 100 and 150 mM provides better protection than 10% DMSO treatment for sperm motility, viability, DNA integrity and mitochondrial functionality. Fertilization and hatching rates were highly correlated (r = 0.95, P < 0.001).
Conclusion:
The addition of 100 - 150 mM trehalose in extender can replace 10% DMSO for the cryopreservation of C. macropomum sperm. doi.org/10.54680/fr22510110312.
