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Photo-Activated Ratiometric Fluorescent Indicator for Real-Time and Visual Detection of Plasma Membrane Homeostasis
Yingcui Bu1, Haoran Wang2, Yu Deng1
1Key Laboratory of Functional Inorganic Materials Chemistry of Anhui University, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University) Ministry of Education, College of Chemistry and Chemical Engineering, Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, P. R. China.
Abstract:
Development of an activated ratiometric indicator that is specific to plasma membrane (PM) viscosity exhibits great application prospects in disease diagnosis and treatment but remains a great challenge. Herein, a photo-activated fluorescent probe (CQ-IC) was designed and prepared tactfully, which could analyze and real-time monitor the microenvironmental homeostasis of the PM based on a two-channel ratiometric imaging model. Interestingly, upon light irradiation, CQ-IC generates reactive oxygen species and thus increases the cellular viscosity, which increases two emission peaks at 480 and 610 nm. This work would propose a new strategy to sensor PM homeostasis and effectively guide the treatment of viscosity-related diseases among various physiological and pathological processes.
