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Updated: Aug 14, 2025
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Published on: December 12, 2011
The effect of different preservation media and temperatures on sperm quality and DNA integrity in mouse cauda
C Yildiz1, N Coskun Cetin2, I Yavas1
1Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Hatay-TURKEY.
Background:
Mouse sperm can be stored for long or short-time periods. Nevertheless long-term storage leds to significantly reduced sperm quality and fertility because of cryodamage. Thus, in the storage of semen in mice, it is necessary to focus on media and temperatures that gives good results in short-term storage.
Objective:
To determine favorable media for short-term storage of mice spermatozoa by evaluating progressive motility, viability, membrane function integrity, acrosome integrity and fragmented DNA rates at various storage temperatures.
Materials And Methods:
Mouse spermatozoa were collected from epididymides of mature CD1 males and samples were stored at 24 degree C and 4 degree C for 60 h.
Results:
Motility, viability and membrane function of mice spermatozoa were greatest when stored in KSOM media. Motility and viability were not different when stored at refrigerator or room temperature in KSOM compared to HTF or PBS mediums for 48 h, but were after 60 h. There was not any significant variation in terms of acrosome integrity in different preservation conditions. Fragmented DNA rates were similar in fresh sperm with KSOM and HTF media, while there was higher damage in PBS medium at 60 h. Overall, sperm parameters were affected significantly by the time of storage and type of preservation medium, and PBS extender was not suitable for mice spermatozoa at room and refrigerated temperatures as it caused the lowest progressive motility, viability, membrane function integrity and the highest DNA damage.
Conclusion:
Mice spermatozoa stored in KSOM retained the best sperm quality parameters both 24 degree C and 4 degree C for the first 48 h. doi.org/10.54680/fr22610110612.
Insights
For short-term mouse sperm storage, KSOM media is superior. Storing mouse spermatozoa in KSOM at 24°C or 4°C maintains optimal quality for up to 48 hours.
Area of Science:
- Reproductive Biology
- Sperm Cryopreservation
- Mammalian Gamete Storage
Background:
- Long-term storage of mouse sperm significantly reduces quality and fertility due to cryodamage.
- Optimizing short-term storage conditions is crucial for maintaining mouse sperm viability and function.
- Identifying suitable media and temperatures is essential for effective short-term semen preservation.
Purpose of the Study:
- To evaluate the efficacy of different media for short-term storage of mouse spermatozoa.
- To assess the impact of storage temperature (24°C and 4°C) on sperm parameters.
- To determine optimal preservation conditions for maintaining progressive motility, viability, membrane integrity, acrosome integrity, and DNA fragmentation.
Main Methods:
- Mouse spermatozoa were collected from mature CD1 male epididymides.
- Sperm samples were stored in various media at 24°C and 4°C for up to 60 hours.
- Key sperm parameters including motility, viability, membrane function, acrosome integrity, and DNA fragmentation were analyzed.
Main Results:
- KSOM media demonstrated superior preservation of motility, viability, and membrane function compared to HTF and PBS.
- Sperm quality in KSOM remained high at both 24°C and 4°C for 48 hours, with significant decline after 60 hours.
- PBS medium resulted in the lowest sperm quality and highest DNA damage, particularly after 60 hours of storage.
Conclusions:
- KSOM is the recommended medium for short-term storage of mouse spermatozoa, preserving key quality parameters effectively.
- Optimal storage duration in KSOM at both tested temperatures is 48 hours to maintain high sperm quality.
- PBS is unsuitable for short-term mouse sperm preservation due to detrimental effects on motility, viability, and DNA integrity.
