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Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector
G Ghosh-Choudhury1, F L Graham
1Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Biochemical and Biophysical Research Communications
|September 30, 1987
Abstract:
A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.