Functionalization of Bacterial Cellulose and Related Surfaces Using a Facile Coupling Reaction by Thermoresponsive...

Recently, bacterial cellulose and related materials attracted significant attention for applications such as leather-like materials, wound healing materials, etc., due to their abundance in pure form and excellent biocompatibility. Chemical modification of bacterial cellulose further helps to improve specific properties for practical utility and economic viability. However, in most cases, chemical modification of cellulose materials involves harsh experimental conditions such as higher temperatures or organic solvents, which may destroy the 3-dimensional network of bacterial cellulose, thereby altering its characteristic properties. Hence, in this work, we have adopted the Suzuki coupling methodology, which is relatively unexplored for chemically modifying cellulose materials. As the Suzuki coupling reaction is tolerable against air and water, modification can be done under mild conditions so that the covalently modified cellulose materials remain intact without destroying their 3-dimensional form. We performed Suzuki coupling reactions on cellulose surfaces using a recently developed thermoresponsive catalyst consisting of poly(N-isopropylacrylamide) (PNIPAM)-tagged N-heterocyclic carbene (NHC)-based palladium(II) complex. The thermoresponsive nature of the catalyst particularly helped to perform reactions in a water medium under mild conditions considering the biological nature of the substrates, where separation of the catalyst can be easily achieved by tuning temperature. The boronic acid derivatives have been chosen to alter the wettability behavior of bacterial cellulose. Bacterial cellulose (BC) obtained from fermentation on a lab scale using a cellulose-producing bacterium called Gluconacetobacter kombuchae (MTCC 6913) under Hestrin-Schramm (HS) medium, or kombucha-derived bacterial cellulose (KBC) obtained from kombucha available in the market or cotton-cellulose (CC) was chosen for the surface functionalization to find the methodology's diversity. Movie files in the Supporting Information and figures in the manuscript demonstrated the utility of the methodology for fluorescent labeling of bacterial cellulose and related materials. Finally, contact angle analysis of the surfaces showed the hydrophobic natures of some functionalized BC-based materials, which are important for the practical use of biomaterials in wet climatic conditions.