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Updated: Aug 14, 2025
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
12.0K
[Advances in thin layer chromatography coupled with mass spectrometry technology]
Xiaowei Zou1, Xing Liu1, Jianming Zhang1
1Shanghai Kezhe Biochemical Technology Co. Ltd., Shanghai 201108, China.
Se Pu = Chinese Journal of Chromatography
|January 12, 2023
Summary
Thin layer chromatography-mass spectrometry (TLC-MS) offers versatile, low-cost analysis. This review classifies TLC-MS interface technologies, highlighting advancements and future needs for high-throughput, real-time monitoring.
Area of Science:
- Analytical Chemistry
- Chromatography
Context:
- Thin layer chromatography (TLC) is a widely used analytical technique due to its simplicity, low cost, and versatility.
- Recent advancements in analytical technology have driven the integration of TLC with mass spectrometry (TLC-MS), emerging as a significant trend.
- The growing demands of the medicine, food, and scientific instrument industries necessitate further development of TLC-MS technology.
Purpose:
- To review and classify TLC-MS interface technologies developed over the past decade.
- To provide a comprehensive overview of different coupling strategies and their associated mass spectrometry ionization sources.
- To identify current bottlenecks and future directions for TLC-MS development.
Summary:
- This study reviews over 150 studies on TLC-MS interface technologies, categorizing them into three strategies: indirect coupling, direct in-situ detection with specialized MS ion sources, and real-time monitoring during TLC development.
- The review details various ionization techniques used in TLC-MS, including FAB-DI, MALDI, SA-LDI, ESI, LIAD/ESI, ESI, EASY-SPE, DSI, and ICP-MS.
- Current challenges include the design and commercialization of "plug and play" components, with future expectations for high-throughput, real-time monitoring, and flexible scanning capabilities.
Impact:
- TLC-MS has significantly improved research efficiency for bioactive substances in food and drugs, particularly when combined with TLC-bioautography.
- The development of standardized, user-friendly TLC-MS components is crucial for broader adoption.
- Future research should focus on comparative studies of desorption/ionization technologies and the implementation of real-time, high-throughput TLC-MS systems.
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