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Updated: Aug 14, 2025
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Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
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Bioactive polysaccharides promote gut immunity via different ways.
Xincheng Wu1, Xiaojun Huang1, Wanning Ma1
1State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides in Jiangxi Province, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China. spnie@ncu.edu.cn.
Food & Function
|January 12, 2023
Summary
Different bioactive polysaccharides modulate gut immunity uniquely. This study compared nine polysaccharides in an immunosuppressed model, revealing distinct mechanisms for T cell regulation, gut microbiota shifts, and metabolite changes, guiding future development.
Area of Science:
- Immunology
- Microbiology
- Biochemistry
Background:
- Bioactive polysaccharides possess intestinal immunomodulatory properties, but their mechanisms vary.
- Understanding these diverse mechanisms is crucial for developing targeted immunotherapies.
Purpose of the Study:
- To compare the distinct intestinal immunomodulatory mechanisms of nine representative bioactive polysaccharides.
- To investigate their effects on T cells, gene expression, metabolome, and gut microbiota in an immunosuppressed model.
Main Methods:
- Utilized a cyclophosphamide (CTX)-induced immunosuppressed mouse model.
- Analyzed T cell counts, transcription factor and inflammatory factor expression, intestinal metabolome, and gut microbiota composition.
- Compared the effects of xanthan gum, inulin, guar gum, arabinogalactan, carrageenan, glucomannan, araboxylan, xylan, and fucoidan.
Main Results:
- Guar gum, inulin, and glucomannan effectively alleviated T cell suppression.
- Inulin promoted beneficial bacteria (Lactobacillus, Monoglobus) and short-chain fatty acid (SCFA) production.
- Fucoidan and carrageenan supported beneficial bacteria (Rikenella, Roseburia) and inhibited harmful bacteria (Helicobacter), while also modulating metabolites.
Conclusions:
- The nine studied polysaccharides exhibit unique mechanisms for promoting intestinal immunity.
- Findings provide a basis for selecting and developing specific polysaccharides for enhanced immunomodulation.
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