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Updated: Aug 14, 2025

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions
Published on: July 22, 2016
An LRR domain-containing membrane protein gene in rotifer Brachionus plicatilis: Sequence feature, expression
Yuxi Wang1, Ying Guo1, Jingjie Hu2
1MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution (Sanya 57202), Ocean University of China, China.
Abstract:
Leucine-rich repeat (LRR) domains mediate multiple innate immune responses via protein-ligand and protein-protein interactions, but their exact roles in invertebrates are poorly understood. Herein, an LRR domain-containing transmembrane protein (BpLRRm) was identified in the rotifer Brachionus plicatilis. The 1069 bp BpLRRm nucleotide sequence contains a 942 bp open reading frame (ORF) encoding a 313 amino acid polypeptide with four LRR motifs harbouring the LXXLXXLXLXXNXLXXL motif, and a transmembrane domain. Treatment with 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) decreased BpLRRm mRNA levels at 3 h, but they increased thereafter and peaked at 12 h. Lipopolysaccharide (LPS) treatment first increased BpLRRm mRNA levels at 3 h, but levels returned to normal at 12 h, then increased and peaked at 24 h. Recombinant BpLRRm protein bound pathogen-related molecular patterns (PAMPs), including LPS, peptidoglycan (PGN), glucan (GLU) and polyinosinic-polycytidylic acid (poly IC), in a dose-dependent manner. Thus, BpLRRm might function as a pattern recognition receptor (PRR) in the innate immunity of B. plicatilis, and mediate responses to environmental pollution.
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