Volatile fatty acids changed the microbial community during feammox in coastal saline-alkaline paddy soil

In order to indicate the effect of volatile fatty acids (VFAs) on the characteristics of feammox and dissimilatory iron reducing bacteria (DIRB) in paddy soils, different VFAs were selected with paddy soils for anaerobic cultivation. Five treatments were set up, respectively, only adding N and both adding N and C (formate + NH₄⁺ (Fo-N), acetate + NH₄⁺ (Ac-N), propionate + NH₄⁺ (Pr-N), and butyrate + NH₄⁺ (Bu-N)) treatments. The concentration of Fe(II), Fe(III), NH₄⁺, and VFAs was assessed within 45 d, and the bacterial community was determined after cultivation. The oxidation rates of NH₄⁺ were the highest in N treatment, while it was the lowest in Fo-N treatment. Under the four C treatments, the consumption of NH₄⁺ and Fe(III) was the fastest in Pr-N treatment, which was consumed by 31.2% and 76.3%, respectively. Different VFAs selected for distinct DIRB. Compared with N treatment, Ac-N and Bu-N treatment increased the relative abundance of DIRB, such as Geobacter and Clostridia, which increased the consumption of VFAs during incubation. Overall, VFAs, especially formate, could promote Fe(III) reduction and compete with the feammox process for the electron acceptors to decrease the feammox reaction, and prohibited soil NH₄⁺ loss. Therefore, VFAs, which was released from organic fertilizer, could reduce NH₄⁺ loss in feammox process of saline-alkaline paddy soils.