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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 23, 2026

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
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Measuring Nonapoptotic Caspase Activity with a Transgenic Reporter in Mice.

P J Nicholls1, Thomas F Pack2,3, Nikhil M Urs2

  • 1Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC 27710 peter.nicholls@duke.edu.

Eneuro
|January 13, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a new tool to measure non-apoptotic caspase activity (NACA) in the brain. This reporter mouse model reveals NACA

Keywords:
amygdalacaspasesin vivo reportermappingnonapoptoticsex differencesstress

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Caspase-3 is crucial for programmed cell death but also plays roles in non-apoptotic processes like neuronal development and plasticity.
  • Non-apoptotic caspase activity (NACA) is vital in neurodevelopment and neural plasticity, yet simple methods for its quantification in vivo are lacking.
  • Existing methods are insufficient for mapping and quantifying NACA in rodent brains, hindering research into its functions.

Purpose of the Study:

  • To develop a simple and effective method for mapping and quantifying non-apoptotic caspase activity (NACA) in vivo.
  • To create a transgenic mouse model with a sensitive fluorescent reporter for caspase activity, localized to the nucleus.
  • To demonstrate the utility of this reporter system in studying NACA's role in neurophysiology and stress responses.

Main Methods:

  • Generation of a transgenic mouse line expressing a novel, highly sensitive, and specific fluorescent reporter for caspase activity.
  • Utilizing the reporter system to investigate NACA's influence on neurophysiology within an amygdalar circuit.
  • Quantifying NACA in the amygdala of mice subjected to restraint stress, analyzing sex-specific differences.

Main Results:

  • The developed transgenic mouse successfully visualizes and quantifies caspase activity, particularly NACA, in vivo.
  • Evidence was obtained demonstrating NACA's influence on the neurophysiology of an amygdalar circuit.
  • A sex-specific, persistent elevation of caspase activity was observed in female mice following restraint stress.

Conclusions:

  • A novel transgenic reporter mouse enables simple and sensitive in vivo mapping and quantification of non-apoptotic caspase activity (NACA).
  • This tool facilitates systems-level investigations into the roles of both apoptotic and non-apoptotic caspase activity in various biological models.
  • The findings highlight sex-specific stress responses mediated by caspase activity in the amygdala, opening avenues for further research.