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Isolation of angiogenin from normal human plasma
R Shapiro1, D J Strydom, K A Olson
1Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Biochemistry
|August 11, 1987
Summary
Angiogenin, a protein that induces blood vessel formation, is found in normal human plasma, not just tumors. This discovery opens new avenues for studying angiogenin
Area of Science:
- Biochemistry
- Molecular Biology
- Protein Chemistry
Background:
- Angiogenin, a protein stimulating blood vessel growth, was initially isolated from human adenocarcinoma cell lines.
- Its presence suggested a potential role in tumor-specific angiogenesis.
Purpose of the Study:
- To investigate the presence and characteristics of angiogenin in normal human plasma.
- To compare plasma-derived angiogenin with tumor-derived angiogenin.
Main Methods:
- Purification of angiogenin from normal human plasma using CM 52 and Mono S cation-exchange chromatography.
- Characterization through analysis of angiogenic and ribonucleolytic activities, amino acid composition, molecular weight, immunoreactivity, and chromatographic behavior.
- Chemical identity confirmation via peptide mapping and sequencing.
Main Results:
- Angiogenin was successfully purified to homogeneity from normal human plasma.
- Plasma-derived angiogenin demonstrated identical physical and functional properties to tumor-derived angiogenin.
- Yields of 60-150 micrograms/L of angiogenin were obtained from plasma or serum.
Conclusions:
- Angiogenin is not exclusively a tumor-specific protein.
- The findings support further research into angiogenin's biological roles, mechanisms of action, and potential diagnostic or prognostic applications.