Fluorescent phenothiazine-fused boron complexes for ratiometric hypochlorite imaging

Fluorescent hypochlorite probes with ratiometric imaging ability are highly desirable for imaging hypochlorite in biological systems. However, it is still challenging to develop new scaffolds for these probes. In this study, we demonstrate that phenothiazine-fused boron complexes are promising scaffolds for the design of ratiometric fluorescent hypochlorite probes. The synthesized complexes based on the scaffold show ultrafast and ratiometric absorption/fluorescence changes for hypochlorite. We also developed an endoplasmic reticulum-targeting probe and demonstrated that it has excellent real-time imaging ability for both endogenous and exogenous hypochlorite in the endoplasmic reticulum of living cells.