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Phorbol esters activate the pathway for phosphatidylethanol synthesis in differentiating HL-60 cells
1McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Biochimica Et Biophysica Acta
|November 12, 1987
Summary
12-O-Tetradecanoylphorbol 13-acetate (TPA) induces unique acidic phospholipid, phosphatidylethanol, synthesis in HL-60 cells. This pathway, mediated by protein kinase C, may play a role in TPA-induced cell differentiation.
Area of Science:
- Cell Biology
- Biochemistry
- Lipid Metabolism
Background:
- 12-O-Tetradecanoylphorbol 13-acetate (TPA) is a known inducer of cellular processes.
- HL-60 cells are a human promyelocytic leukemia cell line commonly used in differentiation studies.
Purpose of the Study:
- To investigate the formation of an unusual acidic phospholipid, phosphatidylethanol, in TPA-treated HL-60 cells.
- To elucidate the mechanism and precursors involved in phosphatidyl alcohol synthesis.
- To explore the potential role of this pathway in TPA-induced HL-60 cell differentiation.
Main Methods:
- Treatment of HL-60 cells with TPA in the presence of ethanol or other alcohols.
- Analysis of lipid synthesis and accumulation.
- Investigation of protein kinase C involvement.
Main Results:
- TPA induces the synthesis of phosphatidylethanol in HL-60 cells, dependent on exogenous ethanol.
- Other alcohols can serve as precursors, forming corresponding phosphatidyl alcohols.
- Protein kinase C activation appears to mediate the phosphatidyl alcohol synthesis pathway.
Conclusions:
- The synthesis of unusual phosphatidyl alcohols is a novel TPA-induced pathway in HL-60 cells.
- Protein kinase C is implicated in the regulation of this lipid synthesis pathway.
- The phosphatidyl alcohol synthesis pathway may be involved in TPA-induced HL-60 cell differentiation.