In vitro human cell-based models to study airway remodeling in asthma
Ying Zhou1, Qirui Duan1, Dong Yang1
1Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shijingshan District, Beijing 100144, China.
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 13, 2023
Summary
This study reviews human cell-based models for investigating airway remodeling in asthma. These models, including cell cultures, organoids, and microfluidic chips, offer alternatives to animal studies for understanding asthma pathology.
Area of Science:
- Pulmonary Medicine
- Cell Biology
- Biomedical Engineering
Background:
- Airway remodeling, characterized by structural changes in the airways, is a key feature of asthma.
- These changes contribute to airway hyperresponsiveness, obstruction, and predict poor patient outcomes.
- In vitro models are crucial for studying airway remodeling as alternatives to animal models.
Purpose of the Study:
- To review current human cell-based models for studying airway remodeling in asthma.
- To discuss the advantages and disadvantages of various in vitro models.
- To explore how these models can advance understanding of asthma pathogenesis.
Main Methods:
- Review of existing literature on in vitro models for airway remodeling.
- Analysis of cell culture techniques, including monocultures and co-cultures.
- Evaluation of emerging technologies like microfluidic chips and organoids.
Main Results:
- Current models include monocultures, co-cultures, microfluidic chips, and organoids.
- Each model type presents unique advantages and limitations for mimicking airway remodeling.
- Advanced models offer greater physiological relevance for studying asthma.
Conclusions:
- Human cell-based in vitro models are vital tools for asthma research.
- Microfluidic chips and organoids show significant promise for accurate disease modeling.
- Further development of these models will enhance our understanding of airway remodeling in asthma.
