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Updated: Aug 14, 2025
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Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
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RBM4 regulates cellular senescence via miR1244/SERPINE1 axis
Luning Wang1, Xiaohong Zhang2, Junxiu Sheng3
1Institute of Cancer Stem Cell, Dalian Medical University, Dalian, 116044, China.
Cell Death & Disease
|January 13, 2023
Summary
The RNA binding protein RBM4 promotes cancer progression by inhibiting cellular senescence. Depleting RBM4 induces senescence and suppresses tumor growth, revealing RBM4 as a potential cancer therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Cellular senescence is a key tumor suppressor mechanism.
- RNA-binding proteins (RBPs) influence cancer progression, but their role in senescence is unclear.
Purpose of the Study:
- To investigate the role of RNA binding protein RBM4 in regulating cellular senescence and cancer progression.
Main Methods:
- Depletion of RBM4 in various cell types, including cancer cells.
- Analysis of SERPINE1 and miR-1244 expression levels.
- In vitro and in vivo cancer progression assays.
- Assessment of senescence induction using SERPINE1 inhibitors and miR-1244 mimics.
Main Results:
- RBM4 depletion induced cellular senescence and inhibited cancer cell proliferation in vitro and in vivo.
- RBM4 knockdown increased SERPINE1 expression by reducing miR-1244 levels.
- miR-1244 directly targets SERPINE1, and RBM4 regulates miR-1244 stability.
- Inhibition of SERPINE1 or mimicry of miR-1244 reversed RBM4 depletion-induced senescence.
Conclusions:
- RBM4 regulates cancer progression by controlling cellular senescence through the miR-1244/SERPINE1 axis.
- This study uncovers a novel mechanism linking RBM4 to senescence.
- RBM4 represents a potential therapeutic target for cancer treatment.
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