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Related Experiment Video

Updated: Aug 14, 2025

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

GOT1 regulates CD8⁺ effector and memory T cell generation.

Wei Xu¹, Chirag H Patel², Liang Zhao³

¹Bloomberg∼Kimmel Institute for Cancer Immunotherapy, Sidney-Kimmel Comprehensive Cancer Research Center, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

|January 14, 2023

Summary

This summary is machine-generated.

Glutamine oxaloacetate transaminase 1 (GOT1) is crucial for CD8+ T cell effector functions, enhancing proliferation and cytotoxicity. Its absence promotes memory CD8+ T cell generation.

Keywords:

CP: Metabolism GOT1 HIF NADH/NAD effector and memory CD8(+) T cell glucose glutamate serine

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Area of Science:

Immunology
Cellular Metabolism
T cell biology

Background:

T cell activation, proliferation, function, and differentiation depend on metabolic regulation.
The role of specific metabolic enzymes in T cell fate remains an active area of research.

Purpose of the Study:

To investigate the role of Glutamine oxaloacetate transaminase 1 (GOT1) in CD8+ T cell metabolism and function.
To elucidate the mechanisms by which GOT1 influences T cell differentiation and effector functions.

Main Methods:

Utilized [U-13C]glucose tracing to track metabolic pathways in T cells.
Employed genetic deletion of GOT1 to assess its impact on T cell populations.
Analyzed T cell proliferation, effector function, and memory cell generation.

Main Results:

GOT1 is critical for CD8+ T cell effector differentiation and function.
GOT1 enhances T cell proliferation by maintaining redox balance and serine-mediated purine biosynthesis.
GOT1 promotes glycolytic programming and cytotoxic activity via posttranslational regulation of HIF protein.
Genetic deletion of GOT1 leads to increased generation of memory CD8+ T cells.

Conclusions:

GOT1 plays a dual role in CD8+ T cell biology, promoting effector functions while its absence favors memory cell development.
Targeting GOT1 could be a strategy to modulate T cell responses for therapeutic applications.