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Updated: Aug 14, 2025
Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue
Published on: January 19, 2019
Optogenetic and chemogenetic manipulation of seizure threshold in mice
Julia A Kravchenko1, Ethan M Goldberg2, Joanna Mattis3
1Department of Neurology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA.
Abstract:
Here, we present a protocol using optogenetics or chemogenetics to assess the neuronal circuits contributing to seizure initiation. Both approaches allow for targeted control of neuronal populations in vivo and can be combined with experimental manipulations to acutely induce seizures in rodent models. We describe how to (1) introduce and (2) activate optogenetic or chemogenetic actuators while (3) inducing seizures via hyperthermia in a mouse model of epilepsy. This protocol can be adapted for use in other induced seizure models. For complete details on the use and execution of this protocol, please refer to Mattis et al. (2022).1.
Insights
This study introduces a protocol using optogenetics or chemogenetics to investigate neuronal circuits involved in seizure initiation. The method allows targeted control of brain cells in vivo to understand epilepsy mechanisms.
Area of Science:
- Neuroscience
- Epilepsy Research
- Optogenetics and Chemogenetics
Background:
- Understanding the specific neuronal circuits that trigger seizures is crucial for developing effective epilepsy treatments.
- Current methods for studying these circuits in vivo are limited in their ability to precisely control neuronal activity.
Purpose of the Study:
- To present a detailed protocol for assessing neuronal circuits contributing to seizure initiation using optogenetics or chemogenetics.
- To demonstrate the combination of these techniques with seizure induction methods in rodent models.
Main Methods:
- The protocol involves introducing and activating optogenetic or chemogenetic actuators in vivo.
- Seizures are acutely induced via hyperthermia in a mouse model of epilepsy.
- This allows for targeted control of specific neuronal populations during seizure onset.
Main Results:
- The described protocol enables precise investigation of neuronal circuit function during seizure initiation.
- Optogenetics and chemogenetics provide powerful tools for manipulating neuronal activity in the context of epilepsy.
Conclusions:
- This protocol offers a robust method for studying the neural basis of seizure initiation.
- The approach is adaptable to various epilepsy models and can advance our understanding of seizure mechanisms.
