An ESIPT-based ratiometric fluorescent probe for detecting H₂O₂ in water environment and biosystems

The outbreak of the COVID-19 has resulted in a great increase in the use of H₂O₂ disinfectant, which is listed as one of the commonly used disinfectants for COVID-19 by the U.S. Environmental Protection Agency. However, excessive use of H₂O₂ disinfectant can threaten human health and damage the water environment. Therefore, it's of great importance to detect H₂O₂ in aquatic environments and biological systems. Herein, we proposed a novel ESIPT ratio fluorescent probe (named probe 1) for detecting H₂O₂ in water environment and biosystems. Probe 1 emits blue fluorescence as the introduction of the phenylboronic acid disrupts the ESIPT process. After reacting with H₂O₂, the phenylboronic acid is oxidatively removed, and the ESIPT process is restored, which makes the fluorescence emission wavelength red-shifted. Probe 1 exhibited a short response time, high sensitivity, and a large Stokes shift to H₂O₂. Importantly, it has been successfully used to detect H₂O₂ not only in actual water samples, but also endogenous and exogenous H₂O₂ in living cells. The characteristics of probe 1 have a wide range of applications in environmental and biological systems.