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FLUIDOT: A Modular Microfluidic Platform for Single-Cell Study and Retrieval, with Applications in Drug Tolerance
Jolien Breukers1,2, Karen Ven1,2,3, Caroline Struyfs4
1Department of Biosystems, Biosensors group, KU Leuven, Willem de Croylaan 42, Leuven, 3001, Belgium.
Small Methods
|January 16, 2023
Summary
FLUIDOT, a new microfluidic platform, enables accessible single-cell analysis and retrieval. It identified drug-tolerant yeast populations and SARS-CoV-2 neutralizing antibodies from COVID-19 patients.
Area of Science:
- Biotechnology
- Microfluidics
- Cell Biology
Background:
- Lab-on-a-chip technologies have advanced single-cell analysis.
- Existing platforms lack accessibility for diverse cell screening and retrieval.
- A versatile and user-friendly platform is needed for comprehensive single-cell studies.
Purpose of the Study:
- Introduce FLUIDOT, a microfluidic platform for accessible single-cell analysis and retrieval.
- Demonstrate the platform's versatility in studying drug tolerance and antibody discovery.
- Enable phenotypic and genotypic analysis for unexplored biological questions.
Main Methods:
- Developed FLUIDOT, a microfluidic platform with customizable microwells, optical tweezers, and cell retrieval.
- Fabricated and operated the FLUIDOT system.
- Validated performance through yeast drug tolerance studies and B cell screening from COVID-19 patients.
Main Results:
- Discovered two distinct drug-tolerant yeast cell populations.
- Identified highly affine, in vitro neutralizing monoclonal antibodies against SARS-CoV-2 from convalescent COVID-19 patients.
- Demonstrated FLUIDOT's ease of fabrication, operation, and accessibility.
Conclusions:
- FLUIDOT offers a versatile, accessible solution for single-cell analysis and retrieval.
- The platform facilitates the study of diverse cell types and downstream analyses.
- FLUIDOT is poised to advance biological research by enabling new discoveries.
