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Identifying effective diagnostic biomarkers and immune infiltration features in chronic kidney disease by

Tao Liu1,2, Xing Xing Zhuang3, Xiu Juan Qin1

  • 1Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

Frontiers in Pharmacology
|January 16, 2023
PubMed

Insights

Researchers identified four key genes, including IL10RA, that are crucial in chronic kidney disease (CKD) development. These genes show potential as diagnostic markers and therapeutic targets for CKD, offering new hope for treatment.

Area of Science:

  • Nephrology
  • Immunology
  • Bioinformatics

Background:

  • Chronic kidney disease (CKD) involves inflammation and immune dysfunction, often progressing to end-stage kidney disease.
  • Current diagnostic markers and therapeutic targets for CKD remain limited.
  • Identifying novel biomarkers and therapeutic strategies is critical for managing CKD.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) in CKD.
  • To explore the functions of DEGs and identify hub genes.
  • To evaluate the diagnostic significance and therapeutic potential of identified hub genes in CKD.

Main Methods:

  • Data from GEO microarrays (GSE104948, GSE116626) were analyzed to find DEGs.
  • Gene Ontology (GO), KEGG, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed.
  • Weighted gene co-expression network analysis (WGCNA), protein-protein interaction networks, and ROC curve analysis were utilized.

Main Results:

  • 657 DEGs were identified, highlighting roles in inflammation and immunomodulation in CKD.
  • Four hub genes (IL10RA, CD45, CTSS, C1QA) were identified through WGCNA and network analysis.
  • IL10RA showed the highest expression and significant correlation with immune cell infiltration and inflammatory pathways; potential drug targets were identified.

Conclusions:

  • IL10RA and other identified hub molecules are implicated in CKD pathogenesis.
  • These hub genes represent potential diagnostic biomarkers for CKD.
  • IL10RA presents a promising target for novel therapeutic interventions in CKD treatment.

Keywords:
bioinformatics analysischronic kidney diseasediagnose biomarkerimmune cell infiltrationmolecular docking

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