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Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening

Yingying Yang1, Chaojie Wu1, Wei Shan1

  • 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Horticulture Research
|January 16, 2023
PubMed

Insights

Mitogen-activated protein kinase (MAPK) MaMPK14 negatively regulates banana fruit ripening by phosphorylating MaMYB4. This phosphorylation enhances MaMYB4

Area of Science:

  • Plant Molecular Biology
  • Fruit Ripening Mechanisms
  • Signal Transduction Pathways

Background:

  • Mitogen-activated protein kinase (MAPK) cascades are vital for plant development and stress responses.
  • The role of MAPK signaling in fruit ripening, particularly in bananas, is not well understood.

Purpose of the Study:

  • To investigate the regulatory mechanism of MAPK in postharvest banana fruit ripening.
  • To elucidate the interaction and function of MaMPK14 and MaMYB4 in banana ripening.

Main Methods:

  • Transient overexpression of MaMPK14 and MaMYB4 in banana fruit.
  • Analysis of gene transcription related to ethylene biosynthesis and fruit softening.
  • In vitro phosphorylation assays to determine MaMPK14's effect on MaMYB4.

Main Results:

  • Overexpression of MaMPK14 and MaMYB4 individually delayed banana fruit ripening, indicating their negative regulatory roles.
  • MaMYB4 was identified as a transcriptional repressor of genes involved in ethylene biosynthesis and fruit softening.
  • MaMPK14 directly phosphorylated MaMYB4 at Ser160, enhancing its stability, binding affinity, and inhibitory function on transcription.

Conclusions:

  • MaMPK14 acts as a negative regulator of banana fruit ripening by phosphorylating MaMYB4.
  • This phosphorylation event strengthens MaMYB4's ability to repress ripening-associated genes.
  • The study reveals a novel MAPK signaling pathway regulating fruit ripening through post-translational modification.

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