Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

838
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
838
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

464
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
464
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.2K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-cost optical system for laser phacoemulsification of cataracts.

Biophotonics discovery·2026
Same author

Comparing multi- and single-exposure speckle contrast optical spectroscopy methods as estimators of blood flow in the diffuse regime.

Journal of biomedical optics·2026
Same author

Real-time tracking of brain oxygen gradients and blood flow during functional activation.

Neurophotonics·2022
Same author

Comparison of functional activation responses from the auditory cortex derived using multi-distance frequency domain and continuous wave near-infrared spectroscopy.

Neurophotonics·2021
Same author

Fast diffuse correlation spectroscopy with a low-cost, fiber-less embedded diode laser.

Biomedical optics express·2021

Related Experiment Video

Updated: Aug 14, 2025

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.7K

Lossless Compressed Sensing of Photon Counts for Fast Diffuse Correlation Spectroscopy.

Arindam Biswas1, Ashwin B Parthasarathy1

  • 1Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, USA.

IEEE Access : Practical Innovations, Open Solutions
|January 16, 2023
PubMed
Summary

A new compressed sensing method for Diffuse Correlation Spectroscopy (DCS) significantly improves photon counting efficiency. This advancement enables faster and more cost-effective deep tissue blood flow measurements using optics.

Keywords:
Biomedical computingbiophotonicsdata compressiondiffuse correlation spectroscopydiffuse opticsoptoelectronic sensorsphoton counting

More Related Videos

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.4K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.6K

Related Experiment Videos

Last Updated: Aug 14, 2025

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.7K
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.4K
High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.6K

Area of Science:

  • Biomedical Optics
  • Optical Instrumentation
  • Signal Processing

Background:

  • Diffuse Correlation Spectroscopy (DCS) is a noninvasive optical technique for measuring deep tissue blood flow.
  • Conventional DCS systems face limitations in photon counting efficiency, especially in low-photon conditions, hindering scalability for large-scale imaging.
  • Bandwidth and processing time are critical challenges for conventional DCS in high-throughput applications.

Purpose of the Study:

  • To introduce a novel, lossless compressed sensing approach for efficient photon count detection in DCS.
  • To overcome the inefficiencies of conventional 32-bit/channel counters in DCS systems.
  • To develop a low-cost, high-speed alternative for deep tissue blood flow measurement.

Main Methods:

  • Implemented a compressed Diffuse Correlation Spectroscopy (DCS) method utilizing binary-coded-decimal counters.
  • Recorded photon counts from 8 channels simultaneously as a single 32-bit number, achieving 87.5% compression efficiency.
  • Validated the compressed DCS approach against conventional DCS using tissue-simulating phantoms and in-vivo human forearm experiments (arm cuff occlusion).

Main Results:

  • The compressed DCS method demonstrated a lossless 87.5% compression efficiency for photon counts.
  • Accurate estimation of tissue blood flow index in phantoms, with no significant difference compared to conventional DCS.
  • Comparable signal-to-noise ratio and dynamic range to conventional DCS for in-vivo blood flow measurements in the human forearm.

Conclusions:

  • The developed lossless compressed sensing DCS approach offers a significant improvement in efficiency and cost-effectiveness.
  • This method meets and exceeds benchmarks set by conventional DCS systems for deep tissue blood flow measurement.
  • Compressed DCS provides a viable low-cost alternative for fast (~100 Hz) optical measurement of deep tissue blood flow.