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Updated: Aug 14, 2025

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Published on: June 27, 2017
An Inducible T7 Polymerase System for High-Level Protein Expression in Diverse Gram-Negative Bacteria
Jennifer L Greenwich1, Melene A Alakavuklar1, Clay Fuqua1
1Department of Biology, Indiana University, Bloomington, Indiana, USA.
Abstract:
A broad host range (BHR)-inducible T7 RNA polymerase system was developed, enabling induction with isopropyl-β-d-thiogalactopyranoside (IPTG), similar to the Escherichia coli strain BL21(DE3) protocol, but it is now applicable in a wide range of bacteria. This system allows for high protein yields and purification from diverse Gram-negative bacteria, including the native host.
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