Electrochemical, Ultrasensitive, and Selective Detection of Nitrite and H₂O₂: Novel Macrostructured Phthalocyanine...

In the current study, the synthesis of tetra-4-(2-methoxyphenoxy) carboxamide cobalt(II) amide-bridged phthalocyanine (CoTMePhCAPc) is described, as well as its characterization by Fourier transform infrared (FT-IR), UV-visible, and mass spectroscopy; powder X-ray diffraction (PXRD); thermogravimetric analysis (TGA); scanning electron microscopy (SEM); and electrochemistry. Sensing of nitrite (NO₂^-) and hydrogen peroxide (H₂O₂) simultaneously was done on CoTMePhCAPc with the composite multiwalled carbon nanotube (MWCNT)-modified glassy carbon electrode (CoTMePhCAPc/MWCNT/GCE) in the range of linear absorption (NO₂^- and H₂O₂: CV 50-750, differential pulse voltammetry (DPV) 50-750, CA 50-500 nmol L^-1), lower detection limit (NO₂^- and H₂O₂: CV 10.5 and 12.5, DPV 10.5 and 11.2, CA 6.0 and 5.5 nmol L^-1), and sensitivity (NO₂^- and H₂O₂: CV 0.379 and 0.529, DPV 0.043 and 0.049, CA 0.033 and 0.040 μA nM^-1 cm^-2). The composite electrode exhibits improved electrocatalytic behavior compared to modified electrodes for nitrite and H₂O₂. The CoTMePhCAPc/MWCNT/GCE sensor displays good selectivity even in the presence of an excess of interfering metal ions and biomolecules at the applied potentials of +400 mV (nitrite) and -400 mV (H₂O₂). Moreover, the fabricated sensor was studied with various phosphate-buffered saline (PBS) (pH 5-9) electrolyte solutions. The unknown H₂O₂ concentration in blood samples and apple juice and nitrite concentration in drinking water and butter leaf lettuce were all measured using the usual addition method. Docking analysis clearly indicates that the ligand shows excellent inhibition activity toward the three subjected protein molecules.