Plasma etching effect on the molecular structure of chitosan-based hydrogels and its biological properties

To reasonably use hydrogels in healthcare field, this study four kinds of chitosan (CTS)-based hydrogels with different molecular structures. With plasma etching, the morphology, chemical groups' proportion, and hydrophilicity of the hydrogel surface were changed. At 40 min of modification, the ratios of CO and NH₂ on the CTS40-based hydrogel surface increased and reached their maximum values of 40.31 % and 89.17 %, respectively. Combined with the changes in hydrophilic chemical groups and the hydrogel's network structure, the hydrogel surface's wettability changed after plasma etching. From the results, CTS40-based hydrogel showed the lowest contact angle (77.40 ± 3.89°) with 80 min modification due to its dense network structure of CTS and appropriate ratio of hydrophilic groups on the surface. With these molecular structural changes, the antibacterial properties of CTS-based hydrogels against Staphylococcus aureus were improved. Moreover, the functional components delivery system coating with these CTS-based hydrogels showed colon-site controlled-release property. The hydrogels also facilitated the growth of Caco2 and Hic cells, which had 72.74 %-453.27 % cell viability of Caco2 cells on the surface. Therefore, the antibacterial property and biocompatibility of plasma modified CTS-based hydrogels have been demonstrated. The mechanism between molecular structure changes of CTS with plasma etching and its properties was discussed, which would provide a promising carrier material for utilizing healthcare field.