Related Experiment Video
Updated: Aug 14, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
Published on: September 2, 2020
Appia: Simpler chromatography analysis and visualization
Richard Posert1,2, Isabelle Baconguis2
1Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, Oregon, United States of America.
Abstract:
Chromatography is an essential family of assays for molecular biology and chemistry. Typically, only a qualitative assessment of peak height, position, and shape are sufficient to proceed. Additionally, chromatography instrument software is proprietary and often locked to a single computer, making data analysis and sharing difficult. Since each manufacturer reports the data in their own proprietary format, performing analysis of experiments which use multiple instruments or sharing data between labs is also challenging. Here we present Appia, a free, open-source chromatography processing and visualization package focused on making analysis, collaboration, and publication quick and easy.
Related Concept Videos
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Affinity Chromatography
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Principles Of Column Chromatography
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...

