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Updated: Aug 14, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Genome sequencing and assembly of Lathyrus sativus - a nutrient-rich hardy legume crop
Sivasubramanian Rajarammohan1, Lovenpreet Kaur1, Anjali Verma1
1National Agri-Food Biotechnology Institute, Mohali, 140306, India.
Abstract:
Grass pea (Lathyrus sativus) is a cool-season legume crop tolerant to drought, salinity, waterlogging, insects, and other biotic stresses. Despite these beneficial traits, this crop is not cultivated widely due to the accumulation of a neurotoxin - β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in the seeds and its association with neurolathyrism. In this study, we sequenced and assembled the genome of Lathyrus sativus cultivar Pusa-24, an elite Indian cultivar extensively used in breeding programs. The assembled genome of Lathyrus was 3.80 Gb in length, with a scaffold N50 of 421.39 Mb. BUSCO assessment indicated that 98.3% of highly conserved Viridiplantae genes were present in the assembly. A total of 3.17 Gb (83.31%) of repetitive sequences and 50,106 protein-coding genes were identified in the Lathyrus assembly. The Lathyrus genome assembly reported here thus provides a much-needed and robust foundation for various genetic and genomic studies in this vital legume crop.
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