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Biosensor for lactate determination in biological fluids. 2. Interference studies.

J Racek1, J Musil

  • 1Department of Clinical Biochemistry, Faculty Hospital, Plzen, Czechoslovakia.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|July 30, 1987
PubMed
Summary
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This study shows a yeast lactate biosensor is specific for lactate determination in biological samples. Adding sodium fluoride eliminates interference from glucose, ensuring accurate lactate measurements.

Area of Science:

  • Biotechnology
  • Biosensor Technology
  • Analytical Chemistry

Background:

  • Lactate determination is crucial in biological and medical diagnostics.
  • Yeast biosensors offer a potential method for lactate quantification.
  • Assessing biosensor selectivity is vital for reliable measurements.

Purpose of the Study:

  • To evaluate the selectivity of a yeast biosensor using Hansenula anomala for lactate detection.
  • To identify potential interfering substances in biological samples like blood plasma.
  • To determine the stability and specificity of the biosensor over time.

Main Methods:

  • Immobilization of aerobic yeast Hansenula anomala cells.
  • Testing the biosensor response to lactate and other metabolites.

Related Experiment Videos

  • Investigating the effect of common blood plasma components (e.g., uric acid, glucose).
  • Evaluating the impact of sodium fluoride on glucose interference.

    • Main Results:

    • The yeast biosensor showed high specificity for lactate, particularly in the initial two weeks.
    • Uric acid caused the highest positive error among reducing substances.
    • Glucose interference was significant but completely eliminated by adding sodium fluoride.
    • Other metabolites present in blood plasma did not cause significant interference.

    Conclusions:

    • The yeast lactate biosensor demonstrates good selectivity for lactate determination in biological materials.
    • Sodium fluoride is effective in mitigating glucose interference, enhancing biosensor reliability.
    • The biosensor is suitable for general use in biological sample analysis for lactate levels.