Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alterexciton: Single-Valley Dipolar Excitons in Noncentrosymmetric Monolayer.

ACS nano·2026
Same author

Unlocking luminescence by decoupling quenching vibrations in Au<sub>21</sub>SR<sub>17</sub> nanoclusters: backbone flexibility <i>versus</i> ligand dynamics.

Nanoscale·2026
Same author

CircUBE3A inhibited endothelium-mesenchymal transition by promoting UBE3A mRNA degradation via ac4C modification in diabetic vascular impairment.

Biochemical pharmacology·2026
Same author

The C2H2 Zinc-Finger protein ELR1 negatively regulates cytokinin metabolism and signaling pathway in rice.

Functional & integrative genomics·2025
Same author

A Redox-Active and Electroactive Hydrogel Enabled by an Integrated PEDOT@PMOF Nanofiller for Post-Infarct Myocardial Repair.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

DPV UL38 stabilizes MFN2 to subvert MAVS-mediated antiviral immunity in ducks.

Veterinary microbiology·2025

Related Experiment Video

Updated: Aug 14, 2025

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties
10:16

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Published on: January 8, 2016

14.0K

Ratiometric fluorescent semiconducting polymer dots for temperature sensing.

Shuyi He1, Steven Wu1

  • 1Department of Chemistry, University of South Dakota, Vermillion, South Dakota, 57069, USA. Steven.Wu01@usd.edu.

The Analyst
|January 18, 2023
PubMed
Summary

This study introduces a novel ratiometric nanoprobe using semiconducting polymer dots (Pdots) and a europium (Eu) complex for precise in vitro temperature sensing. The Pdots-Eu thermometer offers accurate and sensitive temperature monitoring within cells.

More Related Videos

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
07:16

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

6.3K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.5K

Related Experiment Videos

Last Updated: Aug 14, 2025

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties
10:16

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Published on: January 8, 2016

14.0K
Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
07:16

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

6.3K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.5K

Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Semiconducting polymer dots (Pdots) offer advantageous properties like water solubility, light stability, and biocompatibility.
  • Precise temperature monitoring is crucial for various biological and medical applications.

Purpose of the Study:

  • To develop a ratiometric nanoprobe (Pdots-Eu) for accurate in vitro temperature sensing.
  • To utilize the distinct fluorescence properties of Pdots and a europium complex for temperature detection.

Main Methods:

  • Synthesized a ratiometric nanoprobe using poly(9-vinylcarbazole) (PVK), a temperature-sensitive europium (Eu) complex, and polystyrene graft ethylene oxide functionalized with carboxyl groups (PS-PEG-COOH).
  • Investigated the fluorescence emission peaks at 368 nm (PVK) and 611 nm (Eu complex).
  • Evaluated the temperature sensing capability by analyzing the red/blue fluorescence intensity ratio across a temperature range of 25 °C to 55 °C.

Main Results:

  • The Pdots-Eu thermometer exhibited distinct blue and red fluorescence peaks.
  • The red/blue fluorescence intensity ratio decreased linearly with increasing temperature from 25 °C to 55 °C.
  • The nanoprobe demonstrated high sensitivity and accuracy for in vitro temperature detection.

Conclusions:

  • The developed Pdots-Eu ratiometric nanoprobe is effective for precise in vitro temperature monitoring.
  • This technology holds potential for real-time temperature tracking within cellular environments.
  • The ratiometric approach provides a reliable method for temperature sensing in biological systems.