Optimization of extraction tower structure and extraction conditions for lincomycin separation
Related Experiment Video
Updated: Aug 14, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Jinlong Tan1, Junfen Wan1, Saicheng Yu1
1State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, China.
Abstract:
Lincomycin is a widely used aminoglycoside antibiotic. For its separation from fermentation broth in production, solvent extraction is usually applied because of its low cost and high capacity compared to other bioseparation methods. The multistage mixer-settler is a common extraction equipment in commercial production, but it occupies a large area and causes pollution. In this study, a fully enclosed turbine tower was designed and applied in order to replace the mixer-settler. Its structure parameters (turbine diameter, tray porosity) were optimized on the basis of the extraction effect of lincomycin. The results showed that with 35% tray porosity and 28/26 mm turbine diameter of the tower, the extraction rate was kept above 99.0% steadily under 375 rpm/min rotating speed and 60 °C temperature. The extraction effect is much better than mixer-settler and such turbine tower is expected to be applied in the commercial production of lincomycin.
More Related Videos
08:232 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Published on: August 6, 2018
07:10Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes
Published on: September 29, 2023
Related Concept Videos
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Extraction: Advanced Methods
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Elution Process