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Related Experiment Video

Updated: Aug 14, 2025

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
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Hepatitis E Virus: Isolation, Propagation, and Quantification.

Shaoli Lin1, Liping Yang1, Yan-Jin Zhang1

  • 1Molecular Virology Laboratory, Department of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland.

Current Protocols
|January 18, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces improved methods for isolating, propagating, and quantifying Hepatitis E virus (HEV). These advancements aim to enhance the study of HEV, a significant cause of liver disease, especially in pregnant women.

Keywords:
HEVisolationpropagationvirus quantification

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Area of Science:

  • Hepatology and Virology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Hepatitis E virus (HEV) causes acute liver disease through fecal-oral transmission.
  • HEV infection poses a severe risk to pregnant women, with a fatality rate up to 30%.
  • Current cell culture methods for HEV propagation are suboptimal and inefficient.

Purpose of the Study:

  • To present optimized methods for the isolation and propagation of Hepatitis E virus (HEV).
  • To establish efficient protocols for the quantification of HEV.
  • To facilitate further research into HEV biology and pathogenesis.

Main Methods:

  • Isolation and propagation of HEV from clinical specimens in cultured cells.
  • Quantification of HEV RNA using reverse transcription quantitative polymerase chain reaction (RT-qPCR).
  • Recovery of HEV from infectious cDNA clones and subsequent virus purification.
  • Quantification of infectious HEV particles via infectivity assays.

Main Results:

  • Demonstration of effective HEV isolation and propagation techniques.
  • Establishment of reliable methods for HEV RNA and live particle quantification.
  • Improved efficiency in HEV production for research purposes.

Conclusions:

  • The presented methods offer enhanced capabilities for HEV research.
  • These protocols are crucial for advancing the understanding and control of Hepatitis E.
  • Optimized HEV propagation and quantification are vital for developing effective interventions.