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Updated: Aug 14, 2025

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
Neprilysin 4: an essential peptidase with multifaceted physiological relevance
Annika Buhr1, Ronja Schiemann1, Heiko Meyer1,2
1Department of Biology/Chemistry, Zoology and Developmental Biology Section, Osnabruck University, Barbarastrasse 11, D-49076 Osnabruck, Germany.
Abstract:
Neprilysins are highly conserved ectoenzymes that hydrolyze and thus inactivate signaling peptides in the extracellular space. Herein, we focus on Neprilysin 4 from Drosophila melanogaster and evaluate the existing knowledge on the physiological relevance of the peptidase. Particular attention is paid to the role of the neprilysin in regulating feeding behavior and the expression of insulin-like peptides in the central nervous system. In addition, we assess the function of the peptidase in controlling the activity of the sarcoplasmic and endoplasmic reticulum Ca2+ ATPase in myocytes, as well as the underlying molecular mechanism in detail.
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