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Interactome of Paraoxonase PON2 Reveals New Pathways for Tumor Growth Regulation

V D Karlov1,2, N B Pestov1,3,4,5, M I Shakhparonov1

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.

Doklady. Biochemistry and Biophysics
|January 18, 2023
PubMed

Insights

Researchers explored paraoxonase-2 (PON2) interactions, finding it indirectly interacts with epidermal growth factor receptor (EGFR). This suggests PON2 may regulate tumor growth, particularly in cases of mutant EGFR.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Paraoxonase-2 (PON2) is an enzyme with incompletely understood functions.
  • Investigating protein-protein interactions is crucial for elucidating cellular mechanisms.
  • Epidermal growth factor receptor (EGFR) is frequently implicated in tumor development.

Purpose of the Study:

  • To identify novel protein interactors of human PON2.
  • To explore the functional relationship between PON2 and EGFR.

Main Methods:

  • Yeast two-hybrid screening of a cDNA library to identify PON2-interacting proteins.
  • Integration of newly identified interactors with previously published data.

Main Results:

  • Identification of several candidate proteins interacting with PON2.
  • Evidence suggests significant indirect interactions between PON2 and EGFR.
  • A potential regulatory role for PON2 in tumor growth associated with mutant EGFR.

Conclusions:

  • PON2 engages in indirect interactions with EGFR.
  • PON2 may play a role in the regulation of tumor growth involving mutant EGFR.
  • Further studies are warranted to fully elucidate the PON2-EGFR pathway in cancer.
Keywords:
ACAA2CFAP53/CCDC11DCNITM2APON2PPP4R2SFRP4interactomeparaoxonasetwo-hybrid yeast screening

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