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Updated: Aug 14, 2025
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis
Alexa M Jauregui1,2,3, Zoé M Cubero Cortés4, Sean D Meehan1,2,3
1Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Abstract:
Mitochondria participate in many important metabolic processes in the body. The lipid profile of mitochondria is especially important in membrane regulation and pathway signaling. The isolation and study of these lipids can provide unparalleled information about the mechanisms behind these cellular processes. In this chapter, we describe a protocol to isolate mitochondrial lipids from homogenized murine optic nerves. The lipid extraction was performed using butanol-methanol (BUME) and subsequently analyzed using liquid chromatography-mass spectrometry. Further analysis of the raw data was conducted using LipidSearch™ and MetaboAnalyst 4.0.
