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Related Experiment Video

Updated: Aug 14, 2025

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

Protocol and Methods Applicable to Retinal Vascular Diseases.

Saurabh Kumar^1,2, Manjunath B Joshi³, Inderjeet Kaur⁴

¹Prof Brien Holden Eye Research Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India.

Methods in Molecular Biology (Clifton, N.J.)

|January 18, 2023

Summary

This summary is machine-generated.

This study explores lipidomics in ocular diseases, analyzing vitreous humor and tear film using mass spectrometry. It simplifies lipid extraction for better understanding of disease-related lipid alterations.

Keywords:

LC-MS/MS Lipidome profiling Retinal vascular diseases Tear film Vitreous humor

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Last Updated: Aug 14, 2025

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

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Published on: March 12, 2022

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Area of Science:

Biochemistry
Molecular Biology
Ophthalmology

Background:

Lipidomics characterizes lipid classes, with mass spectrometry widely used for lipid profiling.
The human retina is lipid-rich and susceptible to modifications, with alterations linked to retinal diseases.
Understanding lipid involvement in retinal diseases like AMD, DR, and ROP is incomplete.

Purpose of the Study:

To establish a simplified protocol for lipid extraction from ocular fluids.
To analyze lipidomic alterations in vitreous humor and tear film for ocular disease research.
To facilitate qualitative and quantitative lipidome analysis using tandem mass spectrometry.

Main Methods:

Lipid extraction from patient-derived vitreous humor and tear film samples.
Tandem mass spectrometry for qualitative and quantitative lipidome analysis.
Development of a simplified protocol for lipid and metabolite extraction.

Main Results:

A simplified protocol for lipid extraction from ocular fluids was developed.
Qualitative and quantitative lipidome data were obtained from patient samples.
The study provides a method for studying lipid alterations in ocular diseases.

Conclusions:

Vitreous humor and tear film are viable alternatives for studying retinal lipidomics.
The simplified protocol enhances lipid extraction efficiency for mass spectrometry analysis.
This approach aids in understanding lipid alterations in various ocular diseases.