Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

859
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
859
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

674
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
674
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Telemedicine via data glasses in CBRN protection suit-Evaluation of medical qualification and technical feasibility.

PloS one·2026
Same author

The Role of Artificial Intelligence in the Prevention of Intimate Partner Violence and Sexual Violence: A Scoping Review of the Literature.

Journal of sex research·2026
Same author

Lipidomic dataset of optic nerves from the silicone oil-induced ocular hypertension mouse model.

Data in brief·2026
Same author

Phosphatidylserine Decarboxylase Regulates Retinal Ganglion Cell Neurite Outgrowth with Altered Somal Membrane Fluidics and Mitochondrial Morphology.

Biomolecules·2026
Same author

Diabetic Retinopathy as a Risk Factor for Ocular Hypertension or Primary Open Angle Glaucoma in a United States-Based Cohort Study.

Journal of glaucoma·2026
Same author

Phospholipid Interconversion and Transport Are Altered in Glaucoma.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Related Experiment Video

Updated: Aug 14, 2025

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.2K

Lipidomics Analysis with Triple Quadrupole Mass Spectrometry.

Meher A Saleem1,2,3, Anna Mueller1,2, Sanjoy K Bhattacharya4,5

  • 1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2023
PubMed
Summary

Lipidomics uses mass spectrometry to study lipids, essential molecules in cell functions and metabolism. This research details a method for precise lipid analysis, aiding disease state studies.

Keywords:
LipidomicsLipidsMass spectrometryShotgun lipidomicsTriple quadrupole mass spectrometry

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.1K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.3K

Related Experiment Videos

Last Updated: Aug 14, 2025

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.2K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.1K
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.3K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology

Background:

  • Lipids are crucial for cellular functions like signaling, metabolism, and membrane structure.
  • Lipidomics, the study of lipids, offers insights into cellular metabolism and disease states.
  • Shotgun lipidomics is a direct infusion electrospray ionization technique for lipid analysis.

Purpose of the Study:

  • To describe the application of a triple quadrupole mass spectrometer for lipidomics.
  • To demonstrate high sensitivity, accuracy, and precision in lipid analysis using this technique.

Main Methods:

  • Utilizing a TSQ Quantum Access MAX triple quadrupole mass spectrometer.
  • Employing direct-infusion electrospray ionization (ESI) for shotgun lipidomics.
  • Adjusting collision energy, ion mode, and scan type for class-specific lipid identification.

Main Results:

  • The TSQ Quantum Access MAX enables sensitive and accurate lipidomics analysis.
  • Class-specific identification of individual lipids was achieved.
  • The method demonstrated high precision in lipid measurements.

Conclusions:

  • Triple quadrupole mass spectrometry is well-suited for lipidomics.
  • This approach provides a robust method for studying lipid profiles.
  • The described technique supports advancements in understanding lipid roles in health and disease.