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Ultraportable Flow Cytometer Based on an All-Glass Microfluidic Chip.
Jiayu Li1, Yuhan Cui2, Qiucheng Xie2
1School of Life Science, Beijing Institute of Technology, Beijing100081, China.
Analytical Chemistry
|January 19, 2023
Summary
An ultraportable flow cytometer using an all-glass microfluidic chip achieves accurate blood analysis. This miniaturized device enables point-of-care testing, including leukocyte differential counts, for improved diagnostics.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Microfluidics
Background:
- Flow cytometers are essential for biological studies and clinical diagnostics.
- Miniaturization is needed for point-of-care applications, but polymer microfluidics limit accuracy.
- Accurate blood analysis, like leukocyte differential counts, is crucial for health monitoring.
Purpose of the Study:
- To develop an ultraportable flow cytometer (AG-UFCM) for accurate point-of-care blood analysis.
- To overcome the optical limitations of polymer microfluidic chips.
- To enable a four-part leukocyte differential count using a miniaturized flow cytometer.
Main Methods:
- Fabrication of an all-glass microfluidic chip using two-step laser processing.
- Development of an ultraportable flow cytometer with a 90-fold volume reduction.
- Utilizing single fluorescence staining for leukocyte differential counting.
Main Results:
- Achieved a surface roughness of less than 1 nm on the all-glass microfluidic chip.
- Enhanced the signal-to-noise ratio by 3 dB compared to polymer materials.
- Successfully performed a four-part leukocyte differential count with the miniaturized flow cytometer.
Conclusions:
- The all-glass microfluidic chip significantly improves optical performance for miniaturized flow cytometry.
- The developed AG-UFCM is suitable for point-of-care testing, enabling critical blood analyses.
- This work lays the foundation for advanced, portable diagnostic devices.
